Titlebook: Enzymes in Nonaqueous Solvents; Methods and Protocol Evgeny N. Vulfson,Peter J. Halling,Herbert L. Holl Book 2001 Humana Press 2001 biochem

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Karen Heard-Lauréote,Carina Buckleythe importance of retaining native structure is well recognized, potential detrimental changes to the protonation state are often not considered. Usually, it is assumed that the enzyme will exhibit “pH memory” of the previous aqueous solution. As discussed in detail in Chapter 19, this is not always a valid assumption.

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Agile Structures: Scaling Agilitystudied, but the optimal water activity was virtually the same in all solvents (.). Similarly, the optimal water activity was the same when different support materials were used for immobilization of the enzyme but the optimal water concentration varied depending on the water-absorbing capacity of the support (.).

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Microencapsulation of Enzymes and Cells for Nonaqueous Biotransformationsr catalytic activity at synthetically useful levels. In these cases, conventional aqueous-organic two-phase systems are still widely used, although the rates of mass transfer and inactivation of biocatalysts at the interface can be a serious drawback (.-.).

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Very High Activity Biocatalysts for Low-Water Systemsthe importance of retaining native structure is well recognized, potential detrimental changes to the protonation state are often not considered. Usually, it is assumed that the enzyme will exhibit “pH memory” of the previous aqueous solution. As discussed in detail in Chapter 19, this is not always a valid assumption.

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Book 2001ne chemicals, and food ingredients. The high selectivity and mild reaction con- tions associated with enzymatic transformations have made this approach an attractive alternative in the synthesis of complex bioactive compounds, which are often difficult to obtain by standard chemical routes. However,

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