| 书目名称 | Enzyme Engineering | | 副标题 | Methods and Protocol | | 编辑 | James C. Samuelson | | 视频video | | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. .Enzyme Engineering: Methods and Protocols. provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and | | 出版日期 | Book 2013 | | 关键词 | bacterial chromosomes; biological catalyst; enzyme function; gene therapy; protein engineering | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-62703-293-3 | | isbn_softcover | 978-1-4939-6287-7 | | isbn_ebook | 978-1-62703-293-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media New York 2013 |
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发表于 2025-3-21 16:04:59
