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Titlebook: Embryonic Stem Cells; Methods and Protocol Kursad Turksen Book 20021st edition Springer Science+Business Media New York 2002

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书目名称Embryonic Stem Cells
副标题Methods and Protocol
编辑Kursad Turksen
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Embryonic Stem Cells; Methods and Protocol Kursad Turksen Book 20021st edition Springer Science+Business Media New York 2002
描述It is fair to say that embryonic stem (ES) cells have taken their place beside the human genome project as one of the most discussed biomedical issues of the day. It also seems certain that as this millennium unfolds we will see an increase in scientific and ethical debate about their potential utility in society. On the scientific front, it is clear that work on ES cells has already generated new possibilities and stimulated development of new strategies for increasing our und- standing of cell lineages and differentiation. It is not naïve to think that, within a decade or so, our overall understanding of stem cell biology will be as revolutionized as it was when the pioneering hemopoietic stem cell studies of Till and McCulloch in Toronto captured our imaginations in 1961. With it will come better methods for ES and lineage-specific stem cell identification, maintenance, and controlled fate selection. Clearly, ES cell models are already providing opportunities for the est- lishment of limitless sources of specific cell populations. In recognition of the gr- ing excitement and potential of ES cells as models for both the advancement of basic science and future clinical application
出版日期Book 20021st edition
版次1
doihttps://doi.org/10.1385/1592592414
isbn_ebook978-1-59259-241-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2002
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Embryonic Stem Cells as a Model for the Physiological Analysis of the Cardiovascular System, factor (LIF) into cell types of all three germ layers: endoderm, ectoderm and mesoderm (.). However, these differentiation processes occur only when ES cells are cultivated in suspension culture in which they grow to multicellular spheroidal tissues, termed embryoid bodies (EBs).
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Lineage Selection for Generation and Amplification of Neural Precursor Cells,est that ES cell-derived neural system can be used for experimental dissection of various aspects of mammalian neural development. If extended to humans, in vitro-generated neural cells could also be used as source for transplantation-based cell therapy.
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Norbert Pachler,Ben Bachmair,John Cook each . has organ and cell-type specificity and is developmentally controlled. During the cardiomyocytic differentiation of embryonic stem (ES) cells in vitro, the expression of multiple . is differentially regulated, and gap junctional intercellular communication is modulated (.–.).
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Frank H. P. Fitzek,Frank Reichertogenfic protein 4 (BMP4), which suppresses neural differentiation and promotes epidermogenesis. Noggin and chordin bind to BMP with dissociation constants comparable to that of BMP receptors to BMP (.,.). Thus, neural induction in . is controlled by a morphogenetic signaling involving a BMP activity gradient.
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