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Titlebook: ELISA; Theory and Practice John R. Crowther Book 1995 Humana Press 1995

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Politisches und feministisches EngagementThe direct, indirect, and capture ELISAs have now been examined. You should be able to optimize the conditions of the tests and be able to use them to measure antigen or antibody in a variety of formats. Competitive ELISAs involve the principles of all these types of assay.
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Basic Principles of ELISAThis chapter introduces the basic test formats for the performance of solid-phase heterogeneous ELISA. This form of ELISA has been so useful because:
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Theoretical ConsiderationsA level of technical knowledge and expertise is required before any immunoassay can be understood and performed. Tests require basic skills, and it is assumed that workers have acquired some skills through use of laboratory equipment and performance of other test systems.
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Use of Antibodies on Solid-Phase in Capture ELISAIn this exercise, the capture antibody, the detecting antibody, and the conjugate are used at optimal dilutions.
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