Titlebook: Cytoskeleton Dynamics; Methods and Protocol Helder Maiato Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 micr

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Wendy K. Silverman,William M. Kurtinesregate chromosomes. Several model systems have been widely used to dissect the molecular and structural mechanisms behind mitotic spindle assembly and function. These include budding and fission yeasts, which are ideal for genetic and molecular approaches, but show limitations in high-resolution liv

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Clinical Child Psychology Libraryules. We have recently discovered that actin filaments that are embedded inside meiotic spindles (spindle actin) are needed for accurate chromosome segregation in mammalian oocytes. To understand the function of spindle actin in oocyte meiosis, we have developed high-resolution and super-resolution

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Panic Spectrum Disorders and Substance Usee highly regulated assembly and constriction of an actomyosin contractile ring, whose function is to pinch the mother cell in two. Research on the contractile ring has particularly focused on the signaling mechanisms that dictate when and where the ring is formed. In vivo studies of ring constrictio

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Optogenetic Control of Microtubule Dynamics,gh-resolution live-cell microscopy in combination with π-EB1 photodissociation. However, these techniques are broadly applicable to other LOV2-based and likely other blue light-sensitive optogenetics. In addition to being a tool to investigate +TIP functions acutely and with subcellular resolution,

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Measurement of Microtubule Half-Life and Poleward Flux in the Mitotic Spindle by Photoactivation ofsure spindle microtubule dynamics using photoactivation of fluorescently tagged tubulin in living cells. This methodology allows the quantitative discrimination of the turnover of specific microtubule populations (e.g., kinetochore vs. non-kinetochore microtubules), as well as determination of micro

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Knocking Out Multiple Genes in Cultured Primary Neurons to Study Tubulin Posttranslational Modifical enzymes in parallel to obtain a significant change in a given tubulin modification. Here we describe a method to generate primary cells with combinatorial knockout genotypes using conditional knockout mice. The conditional alleles are converted into knockout in the cultured primary cells by transd

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