Titlebook: Clinical Metabolomics; Methods and Protocol Martin Giera,Elena Sánchez-López Book 2025Latest edition The Editor(s) (if applicable) and The

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Distinguishing Artifactual Fatty Acid Dimers from Fatty Acid Esters of Hydroxy Fatty Acids in Untarg that resemble the lipid class fatty acid esters of hydroxy fatty acids (FAHFAs), resulting in misidentification. This chapter details a protocol for LC-MS-based untargeted metabolomics using hydrophilic interaction chromatography (HILIC) that specifically aids in distinguishing artifactual fatty ac

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Advances in Chiral Metabolomic Profiling and Biomarker Discoveryhers have developed various targeted chiral metabolomic methods for the analysis of chiral biomarkers. Here, we highlight the pivotal role of chiral metabolic profiling studies in disease diagnosis, prognosis, and therapeutic interventions. Furthermore, we describe cutting-edge chromatographic and m

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Long Chain Base Profiling with Multiple Reaction Monitoring Mass SpectrometryLCB profile in cells, tissue, and blood. The procedure involves chemical hydrolysis to remove the conjugated headgroups and .-acyl chains, allowing to specifically resolve the underlying LCB structures by LC-MS. This method can also be combined with an isotope labeling approach to determine in vivo

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