Titlebook: Cell Motility and Chemotaxis; Methods and Protocol Carsten Beta,Cristina Martinez-Torres Book 2024 The Editor(s) (if applicable) and The Au

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,Using Multiphoton Intravital Microscopy to Study Neutrophil Transmigration and Blood−Brain Barrier e of interest is exposed through a window allowing imaging of cells in real time. Using MP-IVM, the temporospatial kinetics of leukocyte transendothelial migration can be visualized and quantitated using reporter mice and cell-specific fluorophore-conjugated monoclonal antibodies to track the leukoc

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An In Vivo Metastasis Model Using Genotype-Defined Tumor Organoids,including colorectal cancer (CRC). We previously constructed mouse models that carried major driver mutations of CRC, namely ., ., ., ., and ., in combinations. Comprehensive histological analyses of the models showed a link between mutation combinations and malignant phenotypes, such as invasion, e

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Different Morphotypes of , as Models for Chemotaxis and Locomotion,on. The myxomycete has an astonishing behavioral repertoire and is highly responsive to changes in its environment, which map to changes in its tubular network, internal cytoplasm flow, and cytoskeleton. The behavioral repertoire includes problem-solving, decision-making, and memory. .’s chemo- and

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Flagella and Cell Body Staining of Bacteria with Fluorescent Dyes,ally stained with fluorophores. This chapter describes a flagellar staining protocol with the additional possibility of visualizing the cell body. It offers the opportunity to track conformational changes of flagella and simultaneously track the positions of the cell bodies. The additional use of a

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Repurposing Proximity-Dependent Protein Labeling (BioID2) for Protein Interaction Mapping in ,ndent labeling has proven to be a valuable method for revealing protein-protein interaction networks in living cells. A mutant form of the biotin protein ligase enzyme from . (BioID2) underpins this methodology by producing biotin that is attached to proteins that enter proximity to it. This labels

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Electrotaxis of ,, Migration in an Electric Field,pted in the past to identify the molecular mechanism behind this cellular phenomenon. However, how the cells sense the electric stimulus and transduce it into directed cell migration is still under discussion. Many eukaryotic cells react to applied electric stimulation, including . cells. We use the

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