Titlebook: Current Laboratory Methods in Neuroscience Research; Huangui Xiong,Howard E. Gendelman Book 2014 Springer Science+Business Media New York

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Isolation of Mitochondria from Brain Tissue and Cells

intricacy

Isolation of Synaptosomes from Archived Brain Tissues

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Apraxia

Current Laboratory Methods in Neuroscience Research

TIA742

Brain Tissue Preparation, Sectioning, and Stainingf those methods in neuroscience will be presented herein so students can fully grasp the topic of brain tissue preparation, sectioning, and staining. The selectively introduced methods in this chapter are as follows: (1) Transcardial perfusion, especially on rats and mice, with a supplemental video

健壮

Brain Stereotaxic Injectionctures is extremely important in neuroscience research. Achievement of this goal would not have been possible without the stereotaxic delivery system. This chapter recalls the history of the stereotaxic instrument and development of system applications. In addition, we use the rat coordinate atlas a

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Neuronal Tract Tracing with Light and Electron Microscopy in an enhancer or inhibitor to a chain of neurons in studying specific pathways with molecular biological functionality. The tract tracing, including anterograde, retrograde, and transganglion tract tracing, can be achieved through axon transportation of tracers. Based on the visibility of the trac

LUMEN

Stereological Analysisogy has evolved to keep pace with the ever-changing field of neuroscience. It has moved from its early applications for studying the geologic composition of rocks to an invaluable method to detect both small- and large-scale changes in the complex central nervous system. Recent developments in techn

出没

Preparation and Use of Rodent Hippocampal Sliceslices prepared from rodents have been widely used in electrophysiology and basic neuroscience because of the well-known cytostructures and fiber connections in the hippocampus. To be successful in subsequent experiments, it is essential to prepare the brain slices with minimal damage. This chapter d

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Single-Cell Neuronal Dissociation for Electrophysiological Studieses of ion channels and cell excitability in the varied excitable cells including peripheral and central neurons. Although we understand that many factors can affect the patch-clamp recording, the viability of the peripheral and central neurons is an essential point for the success of the patch-clamp