Titlebook: Coronaviruses and Arteriviruses; Luis Enjuanes,Stuart G. Siddell,Willy Spaan Book 1998 The Editor(s) (if applicable) and The Author(s), un

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The Politics of Love and Struggle,s and the activity of r3CLpro was sensitive to inhibition by serine and cysteine proteinase inhibitors; however, it was not inhibited by EDTA, suggesting that metal ions were not critical for 3CLpro activity.

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Requirement of Proteolytic Cleavage of the Murine Coronavirus MHV-2 Spike Protein for Fusion Activit) at position 757 is responsible for the acquisition of fusogenicity of the MHV-2f S protein. This change also altered the susceptibility to proteolytic cleavage of the MHV-2 S protein which was originally uncleavable. We concluded that the non-fusogenic activity of MHV-2 results from the lack of cleavage of its S protein.

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The Arterivirus Replicaseperties of arteriviruses and coronaviruses as well as their intriguing ancestral relationship at the level of replicase genes, genome organization, and replication strategy (Snijder and Horzinek, 1993; Snijder and Spaan, 1995; Cavanagh, 1997; de Vries ., 1997; Snijder and Meulenberg, 1998).

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The United States Research Satellites cis-acting elements necessary for replication are located in these 5′ and 3′ genomic regions. Furthermore, we inserted the intergenic region of the HCV 229E nucleocapsid protein gene into this basic construct and were able to demonstrate the transcription of “subgenomic” RNAs

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