Titlebook: Computer Optimized Microscopy; Methods and Protocol Elena Rebollo,Manel Bosch Book 2019 Springer Science+Business Media, LLC, part of Sprin

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Automated Macro Approach to Remove Vitelline Membrane Autofluorescence in , Embryo 4D Moviesbryo development. Finally, the fitted selection is used to clear the external glowing halo that, otherwise, would obscure the visualization of the internal embryo labeling upon projection or 3D rendering.

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Photobleaching and Sensitized Emission-Based Methods for the Detection of Förster Resonance Energy Ther..In this chapter several variants of FRET detection methods are described that are based either on the targeted photobleaching of one of the participating molecule species or on the direct detection of the fluorescence signal that is created as a result of the FRET interactions.

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Problems at the Beginning of Life,rics others have been using, we aim at providing the audience with the elementary bricks and methods to build their really own co-localization workflows. A special emphasis is given on the preparatory phase where the acquisition system is assessed, using basic metrological tests.

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Problems in the Care of the Terminally Ill,d usefulness of our proposal, the method was used to compare the traffic of the voltage-gated Kv1.3 potassium channel, which is a well-defined plasma membrane protein, in the presence and absence of KCNE4, a regulatory subunit that strongly retains the channel intracellularly.

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https://doi.org/10.1007/0-306-46801-8 immunostaining, the percentage of cells undergoing different phases of the cell cycle (G0, G1, S, G2, and M) might be established. The method can be also used to estimate the proliferation (S phase) rate of particular cell subpopulations identified through labelling with specific nuclear markers.

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Automated Quantitative Analysis of Mitochondrial Morphologyells from multiple images or tiles. When combined with multiwell plates and automated microscopy, this method may allow to perform high content image analysis of hundreds of cells under different conditions.