| 书目名称 | Chromatin Protocols | | 编辑 | Srikumar P. Chellappan | | 视频video | http://file.papertrans.cn/227/226300/226300.mp4 | | 概述 | Includes new and revised cutting-edge methods and protocols for the study of chromatin.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .Updated and revised, this thorough volume is organized such that it begins with techniques related to the study of chromatin structure. Protocols for reconstitution of chromatin on solid supports for analysis, preparation of positioned mononucleosomes, techniques to study premature chromatin condensation and the use of comparative genomic hybridization to assess genomic aberration are included as well. Novel techniques for imaging chromatin using atomic force microscopy and the isolation of specific genomic regions using engineered DNA binding molecules generated by CRISPR are then examined. That section is followed by protocols to analyze DNA and histone modifications, while the third section includes methods to study DNA replication and repair, in the context of chromatin. Last but not least, protocols for studying chromatin and its relation with transcriptional regulation are presented in a fourth section. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avo | | 出版日期 | Book 2015Latest edition | | 关键词 | Chromatin imaging; Chromatin structure and function; DNA methylation; Histone modifications; Non-histone | | 版次 | 3 | | doi | https://doi.org/10.1007/978-1-4939-2474-5 | | isbn_softcover | 978-1-4939-4406-4 | | isbn_ebook | 978-1-4939-2474-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media New York 2015 |
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