Titlebook: Chloroplast Biotechnology; Methods and Protocol Pal Maliga Book 2014 Springer Science+Business Media New York 2014 Plastid functions.amino

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Plastid Transformation of Tobacco Suspension Cell Culturesed can be laborious, and it can take several months to obtain homoplasmic regenerated plants useful for molecular or physiological studies. In contrast, transformation of tobacco suspension cell plastids provides an easy and efficient system to rapidly evaluate the efficacy of multiple constructs pr

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Tryptophan and Indole Analog Mediated Plastid Transformationedback-insensitive anthranilate synthase (AS) α-subunit gene of tobacco (ASA2) as a selective marker and tryptophan (Trp) or indole analogs as selection agents. AS catalyzes the first reaction in the Trp biosynthetic pathway, naturally compartmentalized in the plastids, by converting chorismate to a

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Plastid Transformation for Rubisco Engineering and Protocols for Assessing Expressionhigher plants the Rubisco large subunit gene, .L, is encoded in the plastid genome, while the Rubisco small subunit gene, .S is coded in the nucleus by a multi-gene family. Rubisco is considered a poor catalyst due to its slow turnover rate and its additional fixation of O. that can result in wastef

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Plastid Transformation in Tomatopecially interesting due to the chloroplast-to-chromoplast conversion occurring during fruit ripening. Moreover, as tomato represents a major food crop with an edible fruit that can be eaten raw, the development of a plastid transformation protocol for tomato was of particular interest to plant biot

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https://doi.org/10.1007/978-981-13-9406-5ntingly poor in other cases. In this review, I summarize our current knowledge about the factors influencing the efficiency of plastid transgene expression and highlight possible optimization strategies to alleviate problems with poor expression levels.

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