Titlebook: Chloroplast Biotechnology; Methods and Protocol Pal Maliga Book 2014 Springer Science+Business Media New York 2014 Plastid functions.amino

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Next-Generation Technologies to Determine Plastid Genome Sequencesdvances in genome sequencing allow the complete sequencing of the chloroplast genome and assessment of variation in the chloroplast genome sequences within a plant. Isolation of chloroplast DNA has been a traditional starting point in these analyses, but the capacity of current sequencing technologi

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Engineering Chloroplasts for High-Level Foreign Protein Expressioncumulation levels arguably being the most attractive one. High-level transgene expression is of particular importance in resistance engineering (e.g., via expression of insecticidal proteins) and molecular farming. Over the past years, the production of many commercially valuable proteins in chlorop

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Excision of Plastid Marker Genes Using Directly Repeated DNA Sequencesplants. The method is simple, efficient, and widely applicable to plants and microalgae. Marker excision frequency is dependent on the length and number of directly repeated sequences. When two repeats are used a repeat size of greater than 600 bp promotes efficient excision of the marker gene. A wi

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Fluorescent Labeling and Confocal Microscopic Imaging of Chloroplasts and Non-green Plastidslization in all tissues of the plants, revealing new features of their morphology and motility. Gene regulatory sequences in plastid transgenes can be optimized through the use of fluorescent protein reporters. Fluorescent labeling of plastids simultaneously with other subcellular locations reveals

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