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Titlebook: Chimera Research; Methods and Protocol Insoo Hyun,Alejandro De Los Angeles Book 2019 Springer Science+Business Media, LLC, part of Springer

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Health Physics Instrumentation,l could grant it protected moral status, making the performance of invasive experiments on the resulting chimera unethical. However, the real ethical problems may involve smaller increases in moral status along what may be termed the “moral staircase” of research subjects, both animal and human. Thr
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Radiation Protection and Dosimetry this chapter we detail the steps for isolating and freezing testicular tissue along with protocols for the subsequent recovery from cryopreservation and transplantation of cells into a recipient testis. Transplantation of cultured or thawed SSCs provides not only a functional assay for identificati
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Informed Consent Issues for Cell Donorsmedical research involving the use of immortal cell lines that carry their donors’ genetic information. The extent and basis for donors’ rights, including the right to withdraw from research, are explored here in detail.
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Chick Models and Human-Chick Organizer Graftsogy necessary for transplantation of human embryonic stem cell (hESC)-derived “gastruloids” stimulated to generate a human organizer into the chick embryo. In these human-chick chimeras, the human organizer cells self-organize to contribute directly to notochord-like tissue and indirectly induce host chick cells to generate neural tissue.
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,Radioactive Atoms–Nature and Behavior, distribution, and vector transduction. This chapter details the procedure for lentiviral transduction and transplantation of human hematopoietic stem/progenitor cells into humanized mice to study inherited human hematological disorders.
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