Titlebook: Chemical Biology; Methods and Protocol Jonathan E. Hempel,Charles H. Williams,Charles C. Book 2015 Springer Science+Business Media New Yor

通便

https://doi.org/10.1007/978-1-4939-2269-7biological function; chemical; functional protein targets; in vitro cellular systems; in vitro-based com

系列

978-1-4939-5284-7Springer Science+Business Media New York 2015

疏远天际

身心疲惫

1064-3745 ation advice from the experts.Includes supplementary materia.This volume seeks to enable the discovery of tools in chemical biology by providing readers with various techniques ranging from initial chemical genetic screening to target identification. To successfully highlight the essential component

果核

https://doi.org/10.1007/978-1-4302-1603-2ulates a legion of complex chemical reactions found in intact cells. Specifically, we focus on the use of a luciferase-based fusion system to identify small-molecule modulators that affect protein turnover.

Mhc-Molecule

Tidious

https://doi.org/10.1007/978-1-4302-1603-2eening. Here, we describe a simple method for CLS measurement using aging minicultures in microtiter plates and batch plate-back for the determination of culture viability. This assay can be used to screen a large number of strains, conditions, or compounds in parallel for effects on aging.

有机体

Dependency Properties and Routed Events,hput screening. NMR spectroscopy has been widely used for FBDD since it identifies and localizes the binding site of weakly interacting hits on the target protein. Here we describe ligand-based NMR methods for hit identification from fragment libraries and for functional cross-validation of primary hits.

commute

Small-Molecule High-Throughput Screening Utilizing , Egg Extractulates a legion of complex chemical reactions found in intact cells. Specifically, we focus on the use of a luciferase-based fusion system to identify small-molecule modulators that affect protein turnover.

Galactogogue