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Titlebook: Cerebrospinal Fluid (CSF) Proteomics; Methods and Protocol Enrique Santamaría,Joaquín Fernández-Irigoyen Book 2019 Springer Science+Busines

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发表于 2025-3-21 20:07:17 | 显示全部楼层 |阅读模式
书目名称Cerebrospinal Fluid (CSF) Proteomics
副标题Methods and Protocol
编辑Enrique Santamaría,Joaquín Fernández-Irigoyen
视频video
概述Includes cutting-edge methods and protocols.provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cerebrospinal Fluid (CSF) Proteomics; Methods and Protocol Enrique Santamaría,Joaquín Fernández-Irigoyen Book 2019 Springer Science+Busines
描述.This volume focuses on protein analysis, including a wide range of the use of mass spectrometry and other protein methods within neurobiological disciplines. Chapters cover topics such as cerebrospinal fluid (CSF) processing and biobanking; label-free quantitative proteomics; SWATH; top-down proteomics; and experimental strategies based on other –omics applied to CSF metabolome, lipidome, and microRNAome. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and thorough, .Cerebrospinal Fluid (CSF) Proteomics: Methods and Protocols. is a valuable resource for graduate students and post-doctoral fellows interested in learning more about CSF proteotyping. It is also useful to established researchers seeking furtherinsight into this growing field. .
出版日期Book 2019
关键词neuroinflammation; Olink technology; biomarkers; QPRESTs; endopeptidome; Proteomics
版次1
doihttps://doi.org/10.1007/978-1-4939-9706-0
isbn_softcover978-1-4939-9708-4
isbn_ebook978-1-4939-9706-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

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发表于 2025-3-21 23:16:07 | 显示全部楼层
Guidelines for CSF Processing and Biobanking: Impact on the Identification and Development of Optimaor disease progression. Cerebrospinal fluid (CSF) is one of the main sources for the identification of novel protein biomarkers for neurological diseases. Despite the enormous efforts employed to identify novel CSF biomarkers, the high variability observed across different studies has hampered their
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Functional Analyses of Embryonic Cerebrospinal Fluid Proteinsuestion to resolve in the field is to precisely describe the eCSF molecules responsible and to understand how these molecules interact in order to exert their functions. Here we describe an in vitro protocol to analyze the influence of eCSF components on neuroepithelium development.
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CSF Sample Preparation for Data-Independent Acquisitiontly used for proteomic-based discovery. In the last years, development and application of mass spectrometry (MS) techniques have made essential contributions to proteomic studies including protein identification as well as quantification. Until recently, biomarker discovery studies were performed th
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Sample Fractionation Techniques for CSF Peptide Spectral Library Generationto acquire essentially all fragment ion spectra in a single LC-ESI-MS/MS experiment. Since the direct correlation between a precursor and its fragment ions is lost when acquiring all ions in a defined ./. range, one data analysis strategy is using so-called peptide spectral libraries. These are usua
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Peptidomic Workflow Applied to Cerebrospinal Fluid Analysis, we studied changes in cerebrospinal fluid (CSF) and blood plasma associated with Guillain-Barre syndrome (GBS)—a rare and severe disorder of the peripheral nervous system with an unknown etiology. Here, we describe the workflow for the analysis of endogenous peptides from CSF. The procedure covers
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Determination of Cerebrospinal Fluid Proteome Variations by Isobaric Labeling Coupled with Strong Ca in the central nervous system (CNS). In this sense, mass spectrometry (MS) methods have proven to be sensitive in quantifying the proteomic profiles of CSF, therefore being able to detect biomarker candidates for neurological disorders. In particular, a key development has been the use of multiplex
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