| 书目名称 | Cell-Free Protein Production | | 副标题 | Methods and Protocol | | 编辑 | Yaeta Endo,Kazuyuki Takai,Takuya Ueda | | 视频video | | | 概述 | Covers cell-free expression systems from E. coli, wheat, insect, and human cells as well as a totally reconstituted system.Shows how cell-free expression can be applied to high-throughput and one-by-o | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .During the past decade as the data on gene sequences and expression patterns rapidly accumulated, cell-free protein synthesis technology has also experienced a revolution, becoming a powerful tool for the preparation of proteins for their functional and structural analysis. In .Cell-Free Protein Production: Methods and Protocols., experts in the field contribute detailed techniques, the uses of which expand deep into the studies of biochemistry, molecular biology, and biotechnology. Beginning briefly with basic methods and historical aspects, the book continues with thorough coverage of protein preparation methods, the preparation of proteins that are generally difficult to prepare in their functional forms, applications of the cell-free technologies to protein engineering, as well as some methods that are expected to constitute a part of future technologies. Written in the highly successful .Methods in Molecular Biology.™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls...Authoritative and cut | | 出版日期 | Book 2010 | | 关键词 | DNA; Laboratory; Microarray; Termination; Translation; membrane proteins; molecular biology; tRNA | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-60327-331-2 | | isbn_softcover | 978-1-4939-5698-2 | | isbn_ebook | 978-1-60327-331-2Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2010 |
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发表于 2025-3-21 17:42:36
