Titlebook: Cell-Free Gene Expression; Methods and Protocol Ashty S. Karim,Michael C. Jewett Book 2022 The Editor(s) (if applicable) and The Author(s),

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Cell-Free Protein Synthesis for High-Throughput Biosynthetic Pathway Prototypingivo or further characterize in vitro. This cell-free pathway prototyping strategy provides a complementary approach to accelerate cellular metabolic engineering efforts toward highly productive strains for metabolite production.

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Cell-Free Synthesis Strategies to Probe Co-translational Folding of Proteins Within Lipid Membranes gradient. This method also includes a protocol to pause and restart translation of membrane proteins at specified positions during their co-translational folding. This stop–start strategy provides an avenue to investigate whether the proteins fold in sequence order, or if the correct fold of N-term

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https://doi.org/10.1057/9781137566287rtificial cells with steady-state dynamics of protein synthesis. Here, we provide the basic procedure for surface patterning, DNA immobilization, capture of protein products on antibody traps and fluorescent imaging. The method of DNA brush surface patterning enables simple parallelization of cell-f

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1064-3745 cal, .Cell‐Free Gene Expression: Methods and Protocols. serves as an idealguide for researchers seeking technical methods to current aspects of CFE and related applications..978-1-0716-2000-7978-1-0716-1998-8Series ISSN 1064-3745 Series E-ISSN 1940-6029

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Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/222943.jpg

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