| 书目名称 | Cell Cycle Synchronization | | 副标题 | Methods and Protocol | | 编辑 | Gaspar Banfalvi | | 视频video | http://file.papertrans.cn/223/222794/222794.mp4 | | 概述 | Offers practical lab protocols for synchronizing mammalian, plant, yeast, fungal, and even bacterial cells.Serves a wide variety of scientists, from cell biologists to medical students to pharmacologi | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In .Cell Cycle Synchronization: Methods and Protocols., experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the “arrest-and-release” approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle. Written in the highly successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, | | 出版日期 | Book 2011 | | 关键词 | Bacteria; Mammalian cells; Model systems; Plant cells; Regulatory mechanisms; Stability; Storage condition | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-61779-182-6 | | isbn_softcover | 978-1-4939-6194-8 | | isbn_ebook | 978-1-61779-182-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC 2011 |
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