Titlebook: Caspases,Paracaspases, and Metacaspases; Methods and Protocol Peter V. Bozhkov,Guy Salvesen Book 2014 Springer Science+Business Media New Y

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Rasheedat M. Mahamood,Esther T. Akinlabirates such as PARP, lamin A, and cytokeratin-18 can be monitored in tissue sections by immunostaining as well as western blots of tissue extracts. In general, more than one method should be used to ascertain detection of activation of caspases in a mouse tissue.

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Springer Series in Advanced Manufacturingactivities of unique caspases are key determinants of the apoptotic process. This chapter describes a set of experimental protocols available for characterization, quantification and inhibition of caspase activities in mammalian cell cultures, including immunoblotting, usage of synthetic substrates, flow cytometry, and microscopic techniques.

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Santiago Gallino,Antonio Morenoof mRNAs, and an increase in cellular adhesion. In lymphocytes, the activity of MALT1 is tightly controlled by its inducible monoubiquitination, which promotes the dimerization of MALT1. Here, we describe both in vitro and in vivo assays that have been developed to assess MALT1 activity.

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