Titlebook: Cancer Systems and Integrative Biology; Usha N. Kasid,Robert Clarke Book 2023 The Editor(s) (if applicable) and The Author(s), under exclu

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Isolation and Identification of Plasma Extracellular Vesicles Protein Biomarkers,V isolation, phase-transfer surfactant method for protein extraction, and mass spectrometry qualitative and quantitative strategies for EV proteome characterization. The pipeline provides a highly effective EV-based proteome analysis technique that can be applied for EV characterization and evaluation of EV-based diagnosis and therapy.

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Genome-Wide Analysis of Palindrome Formation with Next-Generation Sequencing (GAPF-Seq) and a Biointhat are identical to their reverse complements and often arise due to illegitimate repair of DNA double-strand breaks, fusion of telomeres, or stalled replication forks, all of which are common adverse early events in cancer. Here, we describe the protocol for enriching palindromes from genomic DNA

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Sample Preparation and Differential Gene Expression Analysis of Human Cancer Cell Lines by RNA Sequerential gene expression between drug-resistant and sensitive cancers to identify genetic mediators of drug resistance. Here, we describe a comprehensive experimental and bioinformatic pipeline to isolate mRNA from human cell lines, prepare mRNA libraries for next-generation sequencing, and perform

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3D-Autologous Culture Method for Precision Oncology, for the culture medium. 3D-ACM enables tumor cells and/or tissues from an individual patient to proliferate in vitro. in a microenvironment that is very similar to their original, in vivo surroundings. The purpose is to maximally preserve in culture the native biological properties of a tumor. This

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Integration of Single-Cell RNA-Sequencing and Network Analysis to Investigate Mechanisms of Drug Renisms of drug resistance can lead to development of alternative therapies. One strategy is to subject drug-sensitive and drug-resistant variants to single-cell RNA-seq (scRNA-seq) and to subject the scRNA-seq data to network analysis to identify pathways associated with drug resistance. This protoco

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Combination of Tissue Microarray Profiling and Multiplexed IHC Approaches to Investigate Transport py or in combination with other established anticancer or pharmacological agents. To date, nearly a dozen anticancer NAs are approved by the FDA, and several novel NAs are being tested in preclinical and clinical trials for future applications. However, improper delivery of NAs into tumor cells beca

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