Titlebook: Cancer Metabolism; Methods and Protocol Majda Haznadar Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 Human B

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Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical es in cancer cells. Analytical and computational advances are being made constantly, but tissue culture and sample extraction procedures are often variable and not elaborated in the literature. This chapter discusses basic aspects of tissue culture practices as they relate to the use of stable isoto

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Radioluminescence Microscopy: A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes i protocol, we describe how to image radiotracer uptake with single-cell resolution and compare the method to conventional bulk-scale gamma counting. We describe the utility of both techniques, give examples where each technique is recommended, and provide detailed side-by-side instructions for both

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Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-Induced Signalling in Cant. High metabolic and proliferation rate of tumor cells further depletes the local oxygen supply. Therefore, hypoxia is a common phenomenon in multiple solid malignancies. Hypoxia-inducible factors (HIFs) regulate the transcription of a spectrum of genes, which are vitally important for tumor cell

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Determination of Polarization of Resident Macrophages and Their Effect on the Tumor Microenvironmen end, tumor-associated macrophages (TAMs) have been studied extensively and were found to be typically correlated with poor prognosis in various cancers. TAMs are key elements of cancer-associated inflammation promoting cancer progression by increasing angiogenesis, inducing immunosuppression of the

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