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Titlebook: Cancer Cytogenetics; Methods and Protocol Thomas S.K. Wan Book 2017 Springer Science+Business Media, LLC, part of Springer Nature 2017 Arra

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发表于 2025-3-21 18:46:17 | 显示全部楼层 |阅读模式
书目名称Cancer Cytogenetics
副标题Methods and Protocol
编辑Thomas S.K. Wan
视频videohttp://file.papertrans.cn/222/221084/221084.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cancer Cytogenetics; Methods and Protocol Thomas S.K. Wan Book 2017 Springer Science+Business Media, LLC, part of Springer Nature 2017 Arra
描述.This volume provides readers with detailed protocols covering the main cancer cytogenetics techniques needed for clinical utilization and research purposes. The chapters in this book cover topics such as chromosome preparation for myeloid malignancies; chromosome bandings; fluorescence in situ hybridization probe preparation; array-based comparative genomic hybridization; and cytogenetic nomenclature and reporting. The updated reviews on chromosomal abnormalities in hematological malignancies are excellent guides for cytogenetics data interpretations and specific malignant diseases correlation. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and comprehensive, .Cancer Cytogenetics: Methods and Protocols.is a valuable resource for the novice in cytogenetics because it provides helpful guiding protocols, but it’s also great for those who are already engaged in the field and are looking for some technical hints..
出版日期Book 2017
关键词Array Comparative Genomic Hybridization (aCGH); FISH; lymphoid; myelodysplastic syndromes; myeloid leuke
版次1
doihttps://doi.org/10.1007/978-1-4939-6703-2
isbn_softcover978-1-4939-8278-3
isbn_ebook978-1-4939-6703-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2017
The information of publication is updating

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发表于 2025-3-21 20:33:09 | 显示全部楼层
Chromosome Preparation for Myeloid Malignancies,, and disclose subsequent clonal evolution. Three different cell culture methods: direct harvest, nonsynchronized culture, and synchronized culture are usually prepared if the nucleated cell counts in marrow blood are sufficient. Synchronized culture is the first choice of method in myeloid malignan
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Cytogenetic Harvesting of Cancer Cells and Cell Lines,ize the utility of both cell culture—to maximize the yields of the dividing cells needed to harvest mitotic metaphase chromosome preparations and how an empirical evaluation of hypotonic treatments enables optimal conditions to be efficiently determined.
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Chromosome Bandings,oses. Giemsa (G)-, reverse (R)-, and centromere (C)-banding are the most commonly dye-based chromosome-banding techniques. G-banding involves the staining of trypsin-treated chromosomes and R-banding involves denaturing in hot acidic saline followed by Giemsa staining. C-banding is specifically used
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Chromosome Recognition,cisely by recognition of its morphological characteristics and staining patterns according to specific landmarks, regions, and bands as described in the ideogram. Since the quality of metaphases obtained from malignant cells is generally poor for karyotyping, a practical and accurate chromosome reco
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Applications of Fluorescence In Situ Hybridization Technology in Malignancies,and lymphoid malignancies. Cytogenetic analysis has become essential for disease diagnosis, classification, prognostic stratification, and treatment guidance. Fluorescence in situ hybridization (FISH) has greatly enhanced the field and enabled a more precise determination of the presence and frequen
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Fluorescence In Situ Hybridization Probe Preparation, kb to 300 kb, were identified and finely mapped with respect to the Sequence Tagged Site (STS) physical map and with respect to each other. A “golden path” of BACs, covering the entire human genome, was then selected and each clone was fully sequenced. The large number of remaining BACs was not ful
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