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Titlebook: Cancer Cell Biology; Methods and Protocol Sherri L. Christian Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive li

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发表于 2025-3-21 17:31:02 | 显示全部楼层 |阅读模式
书目名称Cancer Cell Biology
副标题Methods and Protocol
编辑Sherri L. Christian
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Cancer Cell Biology; Methods and Protocol Sherri L. Christian Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive li
描述.This volume provides detailed methods on the mechanisms of underlying cancer cell biology. Chapters guide readers through techniques for culturing cancer cell lines, xenografts, cryopreservation of tumor cells, analyzing the co-culture of breast cancer cells, protein secretion by ELISA, flow cytometry-based, multi-parametric immunofluorescence analysis, protein expression by western blot, analysis of surface protein levels, protein recycling by biotinylation assay, and proteomics analysis by liquid chromatography-mass spectrometry. Written in the format of the highly successful .Methods in Molecular Biology .series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols... ..Authoritative and cutting-edge, .Cancer Cell Biology: Methods and Protocols. aims to provide a ..comprehensive set of tools for the analysis of cancer cell biology in the lab..
出版日期Book 2022
关键词Biobanking; chemotaxis assay; Flow-cytometry; CRISPR/Cas; Immunofluorescence
版次1
doihttps://doi.org/10.1007/978-1-0716-2376-3
isbn_softcover978-1-0716-2378-7
isbn_ebook978-1-0716-2376-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

书目名称Cancer Cell Biology影响因子(影响力)




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书目名称Cancer Cell Biology读者反馈学科排名




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发表于 2025-3-22 00:06:25 | 显示全部楼层
https://doi.org/10.1007/978-1-0716-2376-3Biobanking; chemotaxis assay; Flow-cytometry; CRISPR/Cas; Immunofluorescence
发表于 2025-3-22 02:38:19 | 显示全部楼层
978-1-0716-2378-7The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
发表于 2025-3-22 05:52:02 | 显示全部楼层
Sherri L. ChristianIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
发表于 2025-3-22 09:14:48 | 显示全部楼层
Methods in Molecular Biologyhttp://image.papertrans.cn/c/image/221058.jpg
发表于 2025-3-22 16:07:38 | 显示全部楼层
Cancer Cell Biology978-1-0716-2376-3Series ISSN 1064-3745 Series E-ISSN 1940-6029
发表于 2025-3-22 18:59:18 | 显示全部楼层
Nicholas L. Zalewski,Sean J. Pittockancer. Here we describe a method using tandem mass tag (TMT) technology to multiplex up to 18 samples in a single analysis, paving the way for the analysis of large cohorts of tumors, cell lines, and perturbations thereof. The procedure we describe will result in samples ready for in-depth LC-MS/MS analysis in 3–4 days.
发表于 2025-3-22 21:30:15 | 显示全部楼层
Robert L. Sainburg,Pratik K. Muthas and maintaining records on usage and confirmation of identity. Cross-contamination of cancer cell lines has been well documented by the ATCC and must be avoided at all costs. In addition, contamination by . sp. is a well-known issue, which, if left unchecked, can affect all data collected as this
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发表于 2025-3-23 07:57:53 | 显示全部楼层
Volker Dietz,Tobias Nef,William Zev Rymerired to lift the cells from the cell culture vessel for harvesting or subculturing. Many cancer cell lines are adherent, rendering adherent cell culture a critical experimental method in the fields of cell biology, biochemistry, and cancer research. In this chapter, we outline the protocols for cult
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