书目名称 | Cancer Cell Biology | 副标题 | Methods and Protocol | 编辑 | Sherri L. Christian | 视频video | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .This volume provides detailed methods on the mechanisms of underlying cancer cell biology. Chapters guide readers through techniques for culturing cancer cell lines, xenografts, cryopreservation of tumor cells, analyzing the co-culture of breast cancer cells, protein secretion by ELISA, flow cytometry-based, multi-parametric immunofluorescence analysis, protein expression by western blot, analysis of surface protein levels, protein recycling by biotinylation assay, and proteomics analysis by liquid chromatography-mass spectrometry. Written in the format of the highly successful .Methods in Molecular Biology .series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols... ..Authoritative and cutting-edge, .Cancer Cell Biology: Methods and Protocols. aims to provide a ..comprehensive set of tools for the analysis of cancer cell biology in the lab.. | 出版日期 | Book 2022 | 关键词 | Biobanking; chemotaxis assay; Flow-cytometry; CRISPR/Cas; Immunofluorescence | 版次 | 1 | doi | https://doi.org/10.1007/978-1-0716-2376-3 | isbn_softcover | 978-1-0716-2378-7 | isbn_ebook | 978-1-0716-2376-3Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |
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