书目名称 | Calpain | 副标题 | Methods and Protocol | 编辑 | Jeannette S. Messer | 视频video | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .This volume brings together a plethora of protocols and experimental methods used by scientists to study calpains, their inhibitors, and their substrates. It also explores bioinformatic approaches to calpain substrate identification. The chapters in this book are divided into five parts and cover topics such as production and purification of calpains; determination of calpain localization, expression, and activity; identification of calpain-activated protein function; interrogation of calpastatin; and manipulation of calpain expression. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and practical, .Calpain: Methods and Protocols. is a valuable resource for researchers and scientists who want to learn moreabout this developing field, and get inspired to make new discoveries that will aid in diagnosing and treating calpain-related diseases.. | 出版日期 | Book 2019 | 关键词 | proteases; enzymes; Leukocytes; inhibitors; bioinformatic | 版次 | 1 | doi | https://doi.org/10.1007/978-1-4939-8988-1 | isbn_ebook | 978-1-4939-8988-1Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2019 |
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