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Titlebook: Calpain; Methods and Protocol Jeannette S. Messer Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 proteases.en

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发表于 2025-3-21 19:35:33 | 显示全部楼层 |阅读模式
书目名称Calpain
副标题Methods and Protocol
编辑Jeannette S. Messer
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Calpain; Methods and Protocol Jeannette S. Messer Book 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019 proteases.en
描述.This volume brings together a plethora of protocols and experimental methods used by scientists to study calpains, their inhibitors, and their substrates. It also explores bioinformatic approaches to calpain substrate identification. The chapters in this book are divided into five parts and cover topics such as production and purification of calpains; determination of calpain localization, expression, and activity; identification of calpain-activated protein function; interrogation of calpastatin; and manipulation of calpain expression. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Cutting-edge and practical, .Calpain: Methods and Protocols. is a valuable resource for researchers and scientists who want to learn moreabout this developing field, and get inspired to make new discoveries that will aid in diagnosing and treating calpain-related diseases..
出版日期Book 2019
关键词proteases; enzymes; Leukocytes; inhibitors; bioinformatic
版次1
doihttps://doi.org/10.1007/978-1-4939-8988-1
isbn_ebook978-1-4939-8988-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
The information of publication is updating

书目名称Calpain影响因子(影响力)




书目名称Calpain影响因子(影响力)学科排名




书目名称Calpain网络公开度




书目名称Calpain网络公开度学科排名




书目名称Calpain被引频次




书目名称Calpain被引频次学科排名




书目名称Calpain年度引用




书目名称Calpain年度引用学科排名




书目名称Calpain读者反馈




书目名称Calpain读者反馈学科排名




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Bacterial Expression and Purification of Calpainscation of recombinant rat calpain-2 in bacteria was essential for solving the first crystal structures of the calpains in both calcium-free and calcium-bound forms. Here we describe the production and purification of recombinant rat calpain-2 from . using anion-exchange, affinity, and size-exclusion
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In Vivo Monitoring of Calpain Activity by Forster Resonance Energy Transferlated in pathological conditions. Some are ubiquitously expressed (CAPN1, CAPN2, CAPN4, CAPN5, CAPN7, and CAPN10), but others are thought to be localized in specific tissues. The monitoring of in vivo calpain activity is required for physiological, pathological, and therapeutic evaluations. This pas
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Immunohistochemical Localization of Calpains in the Amphibian ssues and provide a large amount of information in a relatively short time and at a low cost. Here we describe methods for indirect immunohistochemistry and immunofluorescence on sections of tadpoles and tissues of adult amphibians belonging to the species . The objective is to localize calpains wit
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Expression and Activity of Calpain A in s cytosol-enriched fractions for measuring calpain activity with the fluorogenic substrate N-LY-AMC. With this method one can measure calpain A activity in wild-type flies and in several mutant fly backgrounds, revealing a strong correlation between in situ membrane distribution and in vitro determi
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