Titlebook: C. elegans; Methods and Applicat Kevin Strange Book 2006 Humana Press 2006 SNP.microscopy.molecular biology.mutant.transcription

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Electrophysiological Analysis of Neuronal and Muscle Function in ,, developed. This chapter will provide an overview of the technical requirements for patch-clamp electrophysiological analysis of . neurons and muscle cells, as well as provide some illustrative examples of insights gained from the pairing of electrophysiological techniques with molecular and genetic analysis.

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Sperm and Oocyte Isolation Methods for Biochemical and Proteomic Analysis,and proteomic applications, including those aiming to identify proteins based on affinity or biological activity. Germline mRNA expression profiles, RNAi feeding clones, and Gateway®-engineered ORFeome vectors are available to help validate experimental results.

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Intracellular pH Measurements In Vivo Using Green Fluorescent Protein Variants,mic, and even behavioral relationships to individual cellular pHi can be inferred. In combination with dye loading of excised or cultured cells, this technique also provides a powerful means of contrasting these relationships to biophysical measurements of ion flux.

POINT

WormBase,dience includes users wishing to query the database beyond the confines of the web interface or fetch data .. No knowledge of programming is necessary or assumed, although users with intermediate skills in the Perl scripting language will be able to utilize additional data-mining approaches.

原始

, Deletion Mutant Screening, categorize the steps needed to do this as follows: (1) mutagenesis and DNA template preparation, (2) PCR detection of deletions, (3) sibling selection, and (4) deletion stabilization. These are discussed in detail in this chapter.

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