Titlebook: Atlas of Human Pluripotent Stem Cells in Culture; Lyn Healy,Ludmila Ruban Book 2015 Springer Science+Business Media New York 2015 embryoni

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Christopher Chase-Dunn,Andrew Jorgensonboth in the setting of quality control and in the assessment of differentiated counterparts. They include mouse embryonic stem cell (mESC) lines, which are routinely grown without feeders (Figs. 12.1, 12.2, 12.3 and 12.4). These cells are easier to maintain than their human counterparts, but they ar

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Mouse and Human Fibroblasts,e cells are isolated from tissues by dissociating the cells using enzymatic treatment and culturing them in the appropriate cell culture medium. These are primary cultures and as such have a limited lifespan. This is the case with both mouse and human fibroblasts.

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Inactivated Mouse and Human Fibroblasts, despite the availability of other systems that are feeder-free. In general, the feeder-based systems tend to be less expensive than their feeder-free equivalents, and feeders have a proven ability to maintain PSCs stably in an undifferentiated state, so in most cases the cost-effective feeder syste

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Human Embryonic Stem Cells,w as colonies and were derived and maintained on mouse feeder cells. The cells displayed a normal karyotype over the reported 8 months in continuous cell culture and displayed cell surface markers characteristic of undifferentiated non-human primate embryonic stem cell lines (Chap. .) and human embr

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