| 期刊全称 | Application of Selected Reaction Monitoring to Highly Multiplexed Targeted Quantitative Proteomics | | 期刊简称 | A Replacement for We | | 影响因子2023 | Michael Kinter,Caroline S. Kinter | | 视频video | | | 发行地址 | Explains both the theoretical and experimental details of the selected reaction monitoring experiment as it is applied to proteins?.Describes a new type of quantitative proteomics experiment called se | | 学科分类 | SpringerBriefs in Systems Biology | | 图书封面 |  | | 影响因子 | A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions. The method that is most widely used is the Western blot analysis. While Western blot is a workhorse in laboratories studying protein expression and has several advantages, it also has a number of significant limitations. In particular, the method is semi-quantitative with limited dynamic range. Western blot focuses on a single protein per sample with only a small number of representative samples analyzed in an experiment. New quantitative tools have been needed for some time to at least supplement, & possibly replace, the Western blot. Mass spectrometric methods have begun to compete with Western blot for routine quantitative analyses of proteins. One of these methods is based on the tandem mass spectrometry technique of selected reaction monitoring (SRM), which is also called multiple reaction monitoring (MRM). Selected reaction monitoring is actually an older tandem mass spectrometry technique, first described in the late 70s, that is widely utilized in the quantitative analysis of | | Pindex | Book 2013 |
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发表于 2025-3-21 17:26:21
