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Titlebook: Agrobacterium Protocols; Volume II Kan Wang Book 2007Latest edition Humana Press 2007 DNA.Flora.algae.arabidopsis thaliana.fungi.genetic en

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Tall Fescue (, Schreb.)d as the selectable marker and hygromycin is used as the selection agent. Calli resistant to hygromycin are obtained after 4–6 wk of selection. Soil-grown tall fescue plants can be regenerated 4–5 mo after .-mediated transformation.
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Agrobacterium Protocols978-1-59745-131-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Tae Hyun Kim,Mark Csikszentmihalyiecalcitrant. Many protocols that previously have been reported were developed for a specific genotype or species. Thus, it has often been necessary to re-optimize a protocol each time research is initiated with a new genotype. The method presented in this chapter has been effective for a wide variety of poplar genotypes.
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,Z, Zai’s Theory of Vital Energy,ted transformation systems are routinely used to engineer cassava. These systems use either somatic embryos or friable embryogenic calli. This paper presents detailed protocols for the transformation of cassava using primary somatic embryos. The effects of explant types, tissue culture conditions, a
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