Titlebook: Aging; Methods and Protocol Sean P. Curran Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 Drosophila.Yeast.li

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Assessing Insulin and Glucose Tolerance in the Aging Mouse,ls. Mice, the most commonly used species to study the biological processes that regulate aging and its associated functional decline, have helped researchers to identify pathways, mechanisms, and genes that regulate aging-related diseases and aging itself and thus are intervention targets. Changes i

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Measurements of Innate Immune Function in ,ation, and protective host responses. Experimentally, . can be infected with a wide variety of human pathogens, as well as with natural pathogens of worms that were isolated from wild-caught nematodes. Here, we focus on an experimental model of bacterial pathogenesis that utilizes the human opportun

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Transcriptional Profiling of , Adult Cells and Tissues with Age,g those that arise with age. Since . is a premier model system used to study the molecular and cellular regulators of adult and aging phenotypes, cell type- and tissue-specific approaches are needed to characterize the genome-wide expression changes associated with these responses. Here we describe

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Assessing Tissue-Specific Autophagy Flux in Adult ,nd has been linked to longevity in many species, including the nematode .. Study of the physiological roles of autophagy during . aging requires methods for the spatiotemporal analysis of autophagy. Here we describe a method for assessing autophagic flux in multiple tissues of . by quantifying the p

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,Staining and Quantification of β-Amyloid Pathology in Transgenic Mouse Models of Alzheimer’s Diseasn of β-amyloid protein (Aβ), which is widely hypothesized to have a key role in AD pathogenesis. Quantification of Aβ in transgenic mice typically is accomplished through both biochemical and histochemical approaches. In this chapter, we describe two techniques for the histological detection of Aβ,

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