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Titlebook: Affinity Biosensors; Techniques and Proto Kim R. Rogers,Ashok Mulchandani Book 1998 Humana Press 1998

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期刊全称Affinity Biosensors
期刊简称Techniques and Proto
影响因子2023Kim R. Rogers,Ashok Mulchandani
视频video
学科分类Methods in Biotechnology
图书封面Titlebook: Affinity Biosensors; Techniques and Proto Kim R. Rogers,Ashok Mulchandani Book 1998 Humana Press 1998
影响因子The frequency of reports concemmg the interface of biological reco- tion elements to signal transduction technologies has risen dramatically over the last decade. Because any one of a wide variety of biological recognition elements (e. g. , antibodies, receptors, DNA, microorganisms, or enzymes) can theoretically be interfaced with any one of a wide variety of signal transducers (e. g. , optical, electrochemical, thermal, or acoustic), the potential range of devices and techniques can be bewildering. The purpose of this volume and the previous volume in this series is to provide a basic reference and startmg point for investigators in academics, mdustry, and government to begin or expand their biosensors research. This volume, Methods in Biotechnology vol. 7: Affinity Biosensors: Techniques and Protocols, describes a variety of classical and emerging transduction technologies that have been interfaced to bioaffinity elements (e. g. , antibodies and receptors). Some of the reasons for the expansion in the use of affinity-based biosensors include both advances in signal transduction technologies (e. g. , fiber optics, microelectromcs, and microfabrication) and the availability of bio
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Immunobiosensors Based on Thermistorsn in the early 1970s. In principle, a chemical or biological process is monitored and quantified by the changes in the thermal signatures of the reacting species. From the fundamental laws in nature governing molecular reactions, virtually all reactions are associated by the absorption or evolution
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Immunosensors Based on Piezoelectric Crystal Deviceelectric microbalances, based on planar microfabrication technique. These devices show a very high sensitivity (up to femtomole) for detecting molecules that are linked to the surface of the device and change its resonant frequency. A sensor surface can be coated selectively for interaction with a s
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Fiberoptic immunosensors with continuous analyte responseical interference, high signal-to-noise ratio with little attenuation over distance, and the capacity to both measure several analytes with fluorescence from a single fiber and bundle fibers without significant crosstalk. For many immunosensors these possibilities have offered little advantage becau
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Immunobiosensors based on grating couplersoaffinity reactions, i.e., of antigen (hapten)-antibody-binding (.–.), but also of receptor—ligand-(.), protein—lipid (., .), and protein—DNA (., .) interactions, and DNA hybridization (.). In each case one of the binding partners is immobilized on the surface of the optical waveguide and binding of
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lmmunobiosensors based on ion-selective electrodesse a simple system for the ISE method to analyze ultratrace amounts of substances in a few microliters of sample solution. This chapter describes a novel and simplified approach for a microliter-ISE system devised by using commercially available conventional ISE without any modification. An essentia
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