Titlebook: Advancing Methods for Biomolecular Crystallography; Randy Read,Alexandre G. Urzhumtsev,Vladimir Y. Lun Conference proceedings 2013 Springe

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Confess

Total Chemical Protein Synthesis for the Determination of Novel X-ray Structures by Racemic Protein∼300 amino acid residues). This in turn enables the preparation of mirror image D-protein molecules not found in nature. Use of a racemic protein mixture (i.e. D-protein+L-protein) greatly facilitates the formation of diffraction-quality crystals of otherwise recalcitrant proteins. Facilitated cryst

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Crystallizing Membrane Proteins for Structure-Function Studies Using Lipidic Mesophases,n the area of G protein-coupled receptors with over a dozen new crystallographic structures emerging in the past 5years. Slowly, it is becoming an accepted method with a proven record and convincing generality. However, it is not a method that is used in every membrane structural biology laboratory

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飞行员

Advanced Applications of Shelxd and Shelxe,IRAS, or MR-SAD. They are command line programs, which is the reason why many people are discourage from using them and exploiting their power. This tutorial shows that shelx c/d/e are not very difficult to use and only little knowledge is required to solve the phase problem and create and initial p

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Angelo Marcello Anile,Vittorio Romano sterics (clashes, H-bonds, packing). All of these both enhance and must be considered along with the information from electron density. The model criteria are primarily local, but their rate of occurrence can also be summarized as a global score.

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Solid Mechanics and Its Applicationsriendly. To this end, tools for handling the mesophase in the pico- to nanolitre volume range have been developed for efficient crystallization screening in manual and robotic modes. Glass crystallization plates have been built that provide unparalleled optical quality and sensitivity to nascent cry

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