Titlebook: Advanced Fluorescence Microscopy; Methods and Protocol Peter J. Verveer Book 2015 Springer Science+Business Media New York 2015 biological

思想

Die ertragsteuerliche Organschaft a brief introduction to the technique, this chapter presents a protocol including background information in order to measure and quantify the molecular interaction of two signaling proteins inside the living cell using fluorescence cross-correlation spectroscopy.

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Overdose

Die ertragsteuerliche Organschaftively interfere in a common focus. This yields a diffraction pattern in the direction of the optical axis, which essentially consists of a main focal spot accompanied by two smaller side lobes. At optimal conditions, the main peak of this so-called point spread function has a full width at half maxi

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Die ertragsteuerliche Organschaftn far-field fluorescence microscopy. The first nanoscopy method successfully applied to conventional biological probes was stimulated emission depletion microscopy (STED). It is based on a physical principle that instantly delivers diffraction-unlimited images, with no need for further computational

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Die ertragsteuerliche Organschaft ~50–100 nm axial resolution. Although such techniques were originally demonstrated on single imaging planes close to the coverslip surface, recent technical developments now enable the 3D imaging of whole fixed cells. We describe methods for converting a 2D PALM into a system capable of acquiring s

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LIMN

Das Schreibwerk beim Zugdienstefamily. A blue light-sensing LOV (light, oxygen, or voltage) domain derived from . (oat) phototropin was fused to the N-terminus of a constitutively active mutant of Rac, via an α-helix (Jα) that is conserved among plant phototropins. The fused LOV domain occluded binding of downstream effectors to

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