期刊全称 | Acoustic Levitation-Based Trace-Level Biosensing | 期刊简称 | Design of Detection | 影响因子2023 | Akihisa Miyagawa | 视频video | http://file.papertrans.cn/144/143972/143972.mp4 | 发行地址 | Nominated as an outstanding Ph.D. thesis by the Tokyo Institute of Technology.Proposes a novel trace detection concept based on a coupled acoustic-gravitational field.Analyzes the kinetics and thermod | 学科分类 | Springer Theses | 图书封面 |  | 影响因子 | This book shows the availability and potential of the coupled acoustic-gravitational (CAG) field for trace-level biosensing. The proposed detection scheme also allows the evaluation of the kinetics and thermodynamics of the reaction occurring on a single microparticle (MP). This method has wide applicability in important fields, involving not only chemistry but also life, environmental, and medical sciences. .The author proposes novel trace-level biosensing based on measurements of the levitation coordinate shift of an MP in the CAG field. The levitation coordinate of the MP in the CAG field is determined by its density and compressibility. The levitation coordinate shift is induced by the binding of gold nanoparticles (AuNPs) to the MP through interparticle reactions. Therefore, the quantity of molecules involved in the reaction can be determined from the levitation coordinate shift. The author demonstrates the zmol level detection for biotin, DNA/RNA, and organic molecules. In addition, the kinetics and thermodynamics are evaluated for various reactions occurring between the MP and AuNP, such as the avidin-biotin reaction, direct hybridization, sandwich hybridization, and aptamer | Pindex | Book 2021 |
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