书目名称 | Drosophila | 副标题 | Methods and Protocol | 编辑 | Christian Dahmann | 视频video | | 概述 | Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | This third edition expands on the previous editions by providing updated protocols and current methods that cover recent breakthroughs in Drosophila research. Chapters guide readers through FlyBase, versatile gene expression systems, analysis of microRNA function, single-cell transcriptome data and metabolism, recent applications of CRISPR for precise genome editing, transcriptional activation and cell lineage tracing, protein inhibition and tagging, optogenetic and optochemical control of tissue mechanics, AFM measurements, sample preparation for electron microscopy, live imaging of different tissues and organs, and quantitative image analysis. Written in the format of the highly successful .Methods in Molecular Biology .series, chapters include an introduction to their topic, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, .Drosophila: Methods and Protocols, Third Edition. serves as a useful and practical guide to new researchers and experts using Drosophila as a model system..Chapter 7 is available open access under a Creative | 出版日期 | Book 2022Latest edition | 关键词 | FlyBase; Gene Expression; CRISPR-Cas9; Optogenetic; Electrophysical recordings; Drosophila melanogaster | 版次 | 3 | doi | https://doi.org/10.1007/978-1-0716-2541-5 | isbn_softcover | 978-1-0716-2543-9 | isbn_ebook | 978-1-0716-2541-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines |
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