旧石器时代 发表于 2025-3-25 03:46:59
Shotgun Proteomics978-1-4939-0685-7Series ISSN 1064-3745 Series E-ISSN 1940-6029Tinea-Capitis 发表于 2025-3-25 09:27:18
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https://doi.org/10.1007/978-1-4939-0685-7High throughput identification; Label-free proteomics; Mass spectrometry; Post-translational modificatiConflagration 发表于 2025-3-25 16:21:35
IPG Strip-Based Peptide Fractionation for Shotgun ProteomicsThe separation of peptides by isoelectric focusing is particularly attractive due to its orthogonality to reverse-phase HPLC. Here, we present a protocol for in-gel peptide isoelectric focusing using immobilized pH gradient strips. The method shows high resolving power for up to 1 mg of sample and is highly reproducible.Liberate 发表于 2025-3-25 20:18:06
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Brain Quantitative Proteomics Combining GeLC-MS and Isotope-Coded Protein Labeling (ICPL) (ICPL) as part of a shotgun proteomics workflow denoting its advantages and disadvantages. This protocol is suitable to studying any proteome of interest, only requiring a specific sample preparation and protein identification. Given our expertise, descriptions here are centered on the study of brain disorders.shrill 发表于 2025-3-26 14:11:38
Employing TMT Quantification in Shotgun-MS Proteomic Analysis: A Focus on Skeletal Muscled even a lesser number quantified, each with its own weaknesses and strengths. Presented here are the “tandem mass tags” isobaric labels (TMT) and a protocol for its utilization in the quantification of skeletal muscle, which might be adapted also to other animal tissues.Cholagogue 发表于 2025-3-26 16:47:49
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