handle
发表于 2025-3-28 18:09:54
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concubine
发表于 2025-3-28 20:46:33
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古老
发表于 2025-3-29 02:25:36
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窗帘等
发表于 2025-3-29 03:29:25
,SHAPE to Probe RNA Structure and RNA–Protein Interactions In Vitro,ranscription reaction. The number of RNA molecules with an adduct at a specific nucleotide position indicates the SHAPE reactivity of that nucleotide. Here, we describe the method for probing the structure of an RNA in a protein-free or a protein-bound state by in vitro SHAPE.
艺术
发表于 2025-3-29 07:28:07
Native RNA Immunoprecipitation (RIP) for Precise Detection and Quantification of Protein-Interactinchain reaction (PCR), microarray or sequencing. Here, we detail a method for native immunoprecipitation, without cross-linking, to isolate protein-RNA complexes followed by subsequent extraction and quantification of the co-purified RNA.
inspired
发表于 2025-3-29 13:52:42
1064-3745 ation advice from the experts.This second edition updates, complements, and expands upon the first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. Chapters explore the isolation and characterization of RNA-pro
干涉
发表于 2025-3-29 18:09:08
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CLASP
发表于 2025-3-29 21:59:24
A Simple Method for the Detection of Wybutosine-Modified tRNAPheGAA as a Readout of Retrograde tRNAmodified tRNA. serves as a readout of retrograde nuclear import and nuclear re-export. This simple assay can be used to determine the role of any gene product in these previously elusive tRNA trafficking events.
meditation
发表于 2025-3-30 02:31:56
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PALSY
发表于 2025-3-30 06:03:38
Global Assessment of Protein Translation in Mammalian Cells Using Polysome Fractionation, gradient and generates the fractions. These fractions can be subjected to further RNA and protein analyses, for example, polysome profiling and mass spectrometry. Here, we present a detailed protocol of polysome fractionation for mammalian cells.