艰苦地移动
发表于 2025-3-25 05:52:42
Analysis of Gene Expression Patterns and RNA Localization by Fluorescence in Situ Hybridization in of a method capable of visualizing a single RNA molecule by utilizing tiled fluorescently labeled oligonucleotide probes that together produce a diffraction-limited spot has greatly increased the resolution of this technique, allowing for the precise determination of subcellular RNA localization and
种子
发表于 2025-3-25 11:24:47
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Heretical
发表于 2025-3-25 12:44:38
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Lacunar-Stroke
发表于 2025-3-25 17:07:38
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感染
发表于 2025-3-25 20:37:09
Chemical Probing of RNA Structure In Vivo Using SHAPE-MaP and DMS-MaP, sulfate (DMS) base reactivity can be employed to investigate the flexibility of nucleotides and correlate it to the constraints imparted by base-pairing and/or protein-binding. In vivo, a multitude of proteins could bind an RNA molecule, regulating its structure and function. Hence, to obtain a mor
fatuity
发表于 2025-3-26 02:32:34
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Mammal
发表于 2025-3-26 08:01:18
Native RNA Immunoprecipitation (RIP) for Precise Detection and Quantification of Protein-Interactin RNA-binding molecules is called RNA immunoprecipitation (RIP). The RNA precipitated with the studied protein can be detected by real-time polymerase chain reaction (PCR), microarray or sequencing. Here, we detail a method for native immunoprecipitation, without cross-linking, to isolate protein-RNA
小争吵
发表于 2025-3-26 12:03:58
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perpetual
发表于 2025-3-26 16:37:49
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genuine
发表于 2025-3-26 16:59:51
Ribosomal Profiling by Gradient Fractionation of Cell Lysates,rful method that is typically employed for monitoring and measuring protein translation status and ribosome activity. Also, it has been used for monitoring the ribosomal stress-responsive events in the ribosome activity. Furthermore, this approach enables understanding of translational regulation, w