含铁
发表于 2025-3-28 15:16:33
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NEX
发表于 2025-3-28 22:08:44
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恩惠
发表于 2025-3-29 02:02:46
1064-3745 ation advice from the experts.This volume provides new technologies on NRPSs and related carrier protein dependent synthases, including polyketide synthases (PKS) and fatty acid synthases (FAS). Chapters detail enzymology, structural biology, proteopromics, chemical biology, natural product chemistr
转折点
发表于 2025-3-29 07:04:47
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liaison
发表于 2025-3-29 08:53:19
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过剩
发表于 2025-3-29 15:17:17
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凹室
发表于 2025-3-29 18:58:34
Chemical Labeling of Protein 4′-Phosphopantetheinylation in Surfactin-Producing Nonribosomal Peptidee chemical labeling of 4′-phosphopantethylated NRPSs. In this chapter, we describe the design and synthesis of an activity-based protein profiling probe and summarize our work toward developing a series of protocols for the labeling and visualization of 4′-phosphopantetheinylation of endogenous NRPSs in complex proteomes.
等待
发表于 2025-3-29 20:37:40
The Assembly-Line Enzymology of Nonribosomal Peptide Biosynthesise chain before releasing the complete peptide. Adenylation, thiolation, condensation, and thioesterase domains play central roles in these reactions. This chapter focuses on the current understanding of these central domains in NRPS assembly-line enzymology.
N防腐剂
发表于 2025-3-30 01:42:48
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modifier
发表于 2025-3-30 04:14:16
Chemoproteomic Profiling of Adenylation Domain Functions in Gramicidin S-Producing Non-ribosomal Pepobust analytical platform for A-domains and provides insights into their enzyme–substrate specificity. In this chapter, we describe the design and synthesis of these ABPP probes and provide a summary of our work on the development of a series of protocols for labeling, visualizing, and analyzing endogenous NRPSs in complex biological systems.