arcane
发表于 2025-3-26 22:42:00
Chemoproteomic Profiling of Adenylation Domain Functions in Gramicidin S-Producing Non-ribosomal Pepdenylation (A) domains in NRPSs are responsible for the incorporation of amino acid building blocks and can be considered as engineering domains; therefore, advanced techniques are required to not only rapidly verify expression and folding, but also accelerate the functional prediction of the A-doma
指令
发表于 2025-3-27 03:36:07
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松紧带
发表于 2025-3-27 07:03:08
Chemo-Enzymatic Synthesis of Non-ribosomal Macrolactams by a Penicillin-Binding Protein-Type Thioestubstrate scopes from the well-exploited ribosomal peptide cyclases and traditional non-ribosomal peptide cyclases. Their unique properties, as well as their stand-alone nature, highlight PBP-type TEs as valuable candidates for development as biocatalysts for peptide macrocyclization. Here in this ch
ACTIN
发表于 2025-3-27 11:45:57
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Irremediable
发表于 2025-3-27 17:11:29
Unraveling Structural Information of Multi-Domain Nonribosomal Peptide Synthetases by Using Photo-Cr serve as important pharmaceutical products. Typically, NRPSs contain one module for the incorporation of one amino acid into the growing peptide chain. A module consists of the domains required for activation, covalent binding, condensation, termination, and optionally modification of the aminoacyl
lesion
发表于 2025-3-27 18:11:40
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dowagers-hump
发表于 2025-3-28 00:21:24
Cross-Linking of the Nonribosomal Peptide Synthetase Adenylation Domain with a Carrier Protein Usingides and related natural products. The A-domain transfers an acyl substrate onto its cognate carrier protein (CP). The proper interactions between an A-domain and the cognate CP are important for functional substrate transfer. To stabilize the transient interactions sufficiently for structural analy
Kindle
发表于 2025-3-28 04:16:01
A Practical Guideline to Engineering Nonribosomal Peptide Synthetasesatural products like scaffolds with changed or improved properties. However, the rational (re-)design of these often gigantic assembly-line proteins is by no means trivial and needs in-depth insights into structural flexibility, inter-domain communication, and the role of proofreading by catalytic d
摇曳的微光
发表于 2025-3-28 07:31:34
Probing Substrate-Loaded Carrier Proteins by Nuclear Magnetic Resonancevalently hold the substrates and intermediates leading to the final product. Thus, how CPs and their partner domains recognize and engage with each other as a function of CP cargos is paramount to understanding and engineering NRPSs. However, rapid hydrolysis of the labile thioester bonds holding su
反感
发表于 2025-3-28 13:03:15
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