江湖郎中 发表于 2025-4-2 22:12:43
Host-Free Systems for Differentiation of Axenic ,fic medium. Shifting promastigotes to this medium, followed by incubation in a CO. incubator, induced differentiation. Axenic amastigotes reach maturation within 5 days, resembling the time it takes in vivo. This chapter provides a complete protocol that should be useful for both Old and New World species of ..