TEN 发表于 2025-3-30 11:35:19

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浮夸 发表于 2025-3-30 15:16:15

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Hyperplasia 发表于 2025-3-30 16:39:44

Targeting Endogenous Loci That Function in , Germline Stem Cells,e other genetic manipulations is useful for studying germline stem cell biology. In this chapter, we will describe a method to use CRISPR-Cas9 genome editing technology to make knock-out and knock-in flies. We will cover everything from guideRNA (gRNA) and donor plasmid design and cloning to screeni

Choreography 发表于 2025-3-31 00:35:37

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Genetics 发表于 2025-3-31 04:00:57

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Exposure 发表于 2025-3-31 08:41:58

Spermatogonial Dedifferentiation into Germline Stem Cells in , Testes,on as a conserved mechanism for replenishing stem cell pools after their loss, thereby maintaining tissue homeostasis. The testis of the fruit fly . offers a simple but powerful system to study dedifferentiation, the process by which differentiating spermatogonia can revert their fate to become full

雄伟 发表于 2025-3-31 12:42:54

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施舍 发表于 2025-3-31 14:11:09

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1FAWN 发表于 2025-3-31 18:16:41

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Heretical 发表于 2025-4-1 00:43:37

A Roadmap for Three-Dimensional Analysis of the Intact Mouse Ovary,n of the dynamic changes occurring at the single-cell level. Here we describe methods for whole-mount immunofluorescence, clearing, imaging, and analysis of whole ovarian tissue in 3D throughout murine development and aging.
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查看完整版本: Titlebook: Germline Stem Cells; Methods and Protocol Michael Buszczak Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under e