TEN 发表于 2025-3-30 11:35:19
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Targeting Endogenous Loci That Function in , Germline Stem Cells,e other genetic manipulations is useful for studying germline stem cell biology. In this chapter, we will describe a method to use CRISPR-Cas9 genome editing technology to make knock-out and knock-in flies. We will cover everything from guideRNA (gRNA) and donor plasmid design and cloning to screeniChoreography 发表于 2025-3-31 00:35:37
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Spermatogonial Dedifferentiation into Germline Stem Cells in , Testes,on as a conserved mechanism for replenishing stem cell pools after their loss, thereby maintaining tissue homeostasis. The testis of the fruit fly . offers a simple but powerful system to study dedifferentiation, the process by which differentiating spermatogonia can revert their fate to become full雄伟 发表于 2025-3-31 12:42:54
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A Roadmap for Three-Dimensional Analysis of the Intact Mouse Ovary,n of the dynamic changes occurring at the single-cell level. Here we describe methods for whole-mount immunofluorescence, clearing, imaging, and analysis of whole ovarian tissue in 3D throughout murine development and aging.