Fretful
发表于 2025-3-25 06:43:13
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Sinus-Rhythm
发表于 2025-3-25 10:03:54
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性行为放纵者
发表于 2025-3-25 13:23:43
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杠杆支点
发表于 2025-3-25 19:14:58
Targeting Endogenous Loci That Function in , Germline Stem Cells,e other genetic manipulations is useful for studying germline stem cell biology. In this chapter, we will describe a method to use CRISPR-Cas9 genome editing technology to make knock-out and knock-in flies. We will cover everything from guideRNA (gRNA) and donor plasmid design and cloning to screening for positive edits.
thyroid-hormone
发表于 2025-3-25 20:49:41
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名义上
发表于 2025-3-26 01:02:51
Derivation and Primordial Germ Cell Induction of Intermediate Pluripotent Stem Cells, PSCs with distinct properties. By modulating the FGF, TGF-β, and WNT pathways, we have derived intermediate PSCs (FTW-PSCs) that are permissive for direct primordial germ cell-like cell (PGC-LC) induction in vitro. Here, we describe the method for derivation and maintenance of mouse and human FTW-PSCs, as well as PGC-LC induction from FTW-PSCs.
START
发表于 2025-3-26 06:56:23
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内向者
发表于 2025-3-26 09:18:36
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窗帘等
发表于 2025-3-26 13:15:18
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TERRA
发表于 2025-3-26 20:48:05
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