incontestable
发表于 2025-3-21 16:53:20
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积习已深
发表于 2025-3-21 22:53:10
https://doi.org/10.1007/978-1-4615-9723-0 results revealed that at least five gRNA sequences, each of them having only one perfectly complementary site in the whole genome, could be designed for more than 95% of genes, regardless of the organism. Next, among those gRNAs, we selected gRNAs that did not have any other complementary site to t
minaret
发表于 2025-3-22 01:19:05
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Indict
发表于 2025-3-22 08:15:28
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IOTA
发表于 2025-3-22 09:41:06
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Mystic
发表于 2025-3-22 14:19:35
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Mystic
发表于 2025-3-22 19:34:07
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放气
发表于 2025-3-22 22:08:14
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minimal
发表于 2025-3-23 02:22:32
Stakeholders, Programmes and Strategies,mizable DNA-binding repeats and nuclease domain of FokI enzyme. Each DNA-binding repeat recognizes one base of double-strand DNA, and functional TALEN can be created by a simple modular assembly of these repeats. To easily and efficiently assemble the highly repetitive DNA-binding repeat arrays, var
Deduct
发表于 2025-3-23 05:46:27
https://doi.org/10.1007/978-1-4615-9723-0the CRISPR/Cas system, the RNA-guided endonuclease Cas protein introduces a DNA double-stranded break at the genome position recognized by a guide RNA (gRNA) based on complementary base-pairing of about 20-nucleotides in length. The 8- or 12-mer gRNA sequence in the proximal region is especially imp