光明正大
发表于 2025-3-26 22:52:47
Designing with Structural CeramicsCRISPR/Cas enables rapid production of genome-edited animals. The Cas9/gRNA component can be introduced to fertilized eggs in several ways. Here, we provide an instructional guide for the generation of knockout mice by cytoplasmic injection using in vitro transcribed Cas9 and gRNA.
FILTH
发表于 2025-3-27 02:50:30
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确定方向
发表于 2025-3-27 06:31:17
CRISPR/Cas9,CRISPR/Cas9 is a novel method that has become the most widely used genome editing technology around the world. Its widespread adoption is largely due to its simplicity and easy of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells expressing the CRISPR/Cas9 system.
monochromatic
发表于 2025-3-27 10:49:34
Genome Editing of Mouse by Cytoplasmic Injection,CRISPR/Cas enables rapid production of genome-edited animals. The Cas9/gRNA component can be introduced to fertilized eggs in several ways. Here, we provide an instructional guide for the generation of knockout mice by cytoplasmic injection using in vitro transcribed Cas9 and gRNA.
玩忽职守
发表于 2025-3-27 17:17:02
Genome Editing of Medaka,Medaka (.), along with zebrafish (.), is a useful experimental model fish. Here, we describe a simple method for generating medaka gene knock-out strains using an automated microchip electrophoresis system. We also describe a method for targeted gene knock-in using a plasmid carrying a sequence that does not cause off-target effects in medaka.
进入
发表于 2025-3-27 20:47:54
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Melanoma
发表于 2025-3-28 01:55:22
Power Analysis and Optimization,we recently established the system for genome editing in embryonic stem (ES) cells and its application for chimeric analysis in mice. In this section, we introduce the procedure for genome editing in mouse zygotes and ES cells.
拍翅
发表于 2025-3-28 03:22:11
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lambaste
发表于 2025-3-28 09:46:03
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Radiation
发表于 2025-3-28 13:55:24
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