柳条筐
发表于 2025-3-21 19:32:42
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形状
发表于 2025-3-21 21:50:25
Defining the Maqasid for Measurementof efficient transformation systems has been hampered by the extreme diversity of ascomycetes, basidiomycetes, and zygomycetes. Polyethylene glycol-assisted transformation of fungal protoplasts is the most widely used system but alternative procedures, e.g., electroporation and .-mediated transforma
perpetual
发表于 2025-3-22 01:31:54
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AUGUR
发表于 2025-3-22 07:34:03
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证实
发表于 2025-3-22 12:48:20
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entrance
发表于 2025-3-22 13:59:39
Intestinal Fluid Handling: Secretion genome technologies, which require handling of very large sized DNA molecules, the spheroplast method has once again become important. We present here the spheroplast method that achieved in the highest transformation efficiency of ..
entrance
发表于 2025-3-22 17:25:38
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积习难改
发表于 2025-3-22 22:40:31
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PLUMP
发表于 2025-3-23 05:20:22
Beyond Subordination: Four Arguments into electro-competent (E-comp) . cells (Becker and Guarente, Methods Enzymol 194:182–187, 1991). Transformation of spheroplasts is more difficult (Cregg et al., Mol Cell Biol 5:3376–3385, 1985), and current methods to make chemically competent . cells results in significantly fewer transformants (
AVID
发表于 2025-3-23 07:04:42
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