发怨言
发表于 2025-3-25 07:24:11
Intestinal Fluid Handling: Secretion genome technologies, which require handling of very large sized DNA molecules, the spheroplast method has once again become important. We present here the spheroplast method that achieved in the highest transformation efficiency of ..
Biguanides
发表于 2025-3-25 08:39:59
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Oligarchy
发表于 2025-3-25 11:44:41
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反话
发表于 2025-3-25 19:32:33
https://doi.org/10.1007/978-1-4471-0471-1ng the time-consuming repetitive purification of protoplasts used for the widely applied polyethylene glycol-mediated method. Biolistic transformation is also relatively quick compared to other available methods and provides a high percentage of stable transformants.
Ledger
发表于 2025-3-25 19:59:01
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征服
发表于 2025-3-26 02:28:12
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GROVE
发表于 2025-3-26 06:51:01
Biolistic Transformation of , for Homoplasmic Mitochondrial Genome Transformantss located on the genome and many important metabolism functions associated with mitochondria, such as energy metabolism, generation of reactive oxygen species. Here, we describe a method to transform the mitochondrial genome of . by biolistic transformation.
收集
发表于 2025-3-26 11:23:47
Use of the Biolistic Particle Delivery System to Transform Fungal Genomesng the time-consuming repetitive purification of protoplasts used for the widely applied polyethylene glycol-mediated method. Biolistic transformation is also relatively quick compared to other available methods and provides a high percentage of stable transformants.
葡萄糖
发表于 2025-3-26 15:00:59
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admission
发表于 2025-3-26 17:35:56
Mediated Transformationzing the AMT process with new fungal species. The chapter also includes a highly efficient vector construction system based on Uracil Specific Excisions Reagent (USER) cloning and specific PCR generated building blocks, which can be combined ad hoc to create complex plasmids in a single cloning step.