弄皱
发表于 2025-3-30 08:32:29
Transformation Methodsy metabolites with importance in pharmaceutical industry or in the biocontrol of significant phytopathogenic fungi (Cardoza et al., Curr Genet 34:50–59, 2005; Sivasithamparam and Ghisalberti, . and ., London, pp 139–191). Other . species produce enzymes with a remarkable industrial importance and ev
Pigeon
发表于 2025-3-30 12:22:03
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Fraudulent
发表于 2025-3-30 19:18:01
Transformation of ,: Spheroplast Method genome technologies, which require handling of very large sized DNA molecules, the spheroplast method has once again become important. We present here the spheroplast method that achieved in the highest transformation efficiency of ..
Ischemic-Stroke
发表于 2025-3-30 21:16:52
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直觉没有
发表于 2025-3-31 04:40:19
Chemical Transformation of ,ation-techniques, the lithium acetate-based protocol described below is easy to perform, requires basic lab equipment and reliably yields good number of the expected transformants. This method can also be adapted for high-throughput analysis purposes.
Cuisine
发表于 2025-3-31 08:48:13
Electroporation of , into electro-competent (E-comp) . cells (Becker and Guarente, Methods Enzymol 194:182–187, 1991). Transformation of spheroplasts is more difficult (Cregg et al., Mol Cell Biol 5:3376–3385, 1985), and current methods to make chemically competent . cells results in significantly fewer transformants (
吸气
发表于 2025-3-31 12:20:17
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