burgeon
发表于 2025-3-21 18:02:08
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亚当心理阴影
发表于 2025-3-21 21:45:29
Construction of Gene-Targeting Vectors by Recombineeringof knockout vectors has been greatly facilitated by recombineering as it allows one to choose any genomic region to manipulate. We describe here an efficient recombineering-based protocol for making mouse conditional knockout targeting vectors.
透明
发表于 2025-3-22 02:47:26
Gene-Trap Vectors and Mutagenesish-throughput use, in an effort to inactivate all genes in the mouse genome. Gene trapping is performed with vectors that simultaneously inactivate and report the expression of the trapped gene and provide a molecular tag for its rapid identification. Gene-trap approaches have been used successfully
运动的我
发表于 2025-3-22 05:19:42
Chromosome Engineering in ES Cellsr phenotypically neutral. To understand the genetic consequences of such genomic changes, these mutations need to be modelled in experimentally tractable systems. The mouse is an excellent organism for this analysis because of its biological and genetic similarity to humans, the ease with which its
ingenue
发表于 2025-3-22 11:22:30
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愤愤不平
发表于 2025-3-22 16:17:17
Generation of shRNA Transgenic Miceeasy manner. For the use of RNAi in mice, short hairpin (sh) RNAs expressed stably from the genome are a faster alternative to conventional knockout approaches. Here, we describe an advanced strategy for complete or conditional gene knockdown in mice, where the Cre/loxP system is used to activate RN
愤愤不平
发表于 2025-3-22 19:30:09
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geometrician
发表于 2025-3-22 23:18:15
Gene Targeting in Mouse Embryonic Stem Cellsr ability to alter the mouse genome has been limited by both the lack of technologies to conditionally target a locus and by conventional cloning. The “cre/loxP” and “recombineering” technologies have overcome some of these limitations and have greatly enhanced our ability to manipulate the mouse ge
钩针织物
发表于 2025-3-23 05:20:19
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patriot
发表于 2025-3-23 08:27:25
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