独行者 发表于 2025-3-26 22:02:26

Visualization of , Long Noncoding RNA with a Fluorescent CRISPR/Cas9 System,PR/Cas9-based approach. This strategy is relatively simple approach to track . at different stages of cell differentiation, providing mechanistic insights into the initiation, maintenance, and establishment of X inactivation.

implore 发表于 2025-3-27 03:54:55

http://reply.papertrans.cn/32/3134/313386/313386_32.png

novelty 发表于 2025-3-27 06:08:11

http://reply.papertrans.cn/32/3134/313386/313386_33.png

demote 发表于 2025-3-27 10:05:14

https://doi.org/10.1007/978-3-322-98482-1ofile m.A along the transcriptome. We present here a protocol for m.A crosslinking immunoprecipitation sequencing (m.A-CLIP-seq), which profiles m.A on mRNA at high resolution from as little as 1 μg of poly(A)-selected mRNA.

needle 发表于 2025-3-27 15:56:04

Ewald Balcar,Stephen W. Loveseyn-reagent, as exemplified on a 110 nucleotide RNA obtained via in vitro transcription. The method exploits the fact that, under neutral buffer conditions (~pH 6.8), the 5′-phosphate carries the only mildly acidic proton in the RNA molecule, which allows for selective functionalization at that site using diazo reagents.

同步左右 发表于 2025-3-27 18:24:46

http://reply.papertrans.cn/32/3134/313386/313386_36.png

QUAIL 发表于 2025-3-28 01:08:33

Effects of Air Pollutants on Vegetation,bisulfite sequencing (RNA-BisSeq). Here we introduce an optimized RNA-BisSeq method by using ACT random hexamers to prime the reverse transcription of bisulfite-treated RNA samples to detect the m.C sites.

inspiration 发表于 2025-3-28 02:31:19

http://reply.papertrans.cn/32/3134/313386/313386_38.png

Mettle 发表于 2025-3-28 07:41:43

http://reply.papertrans.cn/32/3134/313386/313386_39.png

mendacity 发表于 2025-3-28 12:14:09

http://reply.papertrans.cn/32/3134/313386/313386_40.png
页: 1 2 3 [4] 5 6 7
查看完整版本: Titlebook: Epitranscriptomics; Methods and Protocol Narendra Wajapeyee,Romi Gupta Book 2019 Springer Science+Business Media, LLC, part of Springer Nat